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Ambystomatidae is a family of salamanders commonly known as the mole salamanders. Most of the species within this family are characterised by polyandrous mating, in which females mate with and can lay clutches fathered by multiple males. The extent of polyandrous mating is thought to vary based on environment and with the occurrence of parental care, which varies across the genus Ambystoma. The extent of polyandrous mating can be explored using parentage analysis to determine how many males father a female’s clutch. This study screens numerous microsatellite markers that have been identified and used for other species in the genus Ambystoma to determine their usefulness in the species Ambystoma annulatum, so that it may eventually be compared to other species in the genus.
